Decoding Cellular Phenotypes in Label-Free Nonlinear Imaging of Living Tissues
Submitted to SPIE Photonics West 2027 (Multiphoton Microscopy), 2027
Characterization of protein-defined cellular phenotypes in living lymph-node tissue through label-free third-harmonic generation imaging. Integration of multiplexed immunofluorescence with three-dimensional imaging resolves more than 20 immune-cell subtypes and reveals their spatial organization, migratory behavior, and metabolic states without exogenous labels.
